If you find something fun to do in the mountains, you can do it. There are no contents locked away until you reach a certain level. Unlike other sports game apps-and even other genres-there are no mandatory challenges that you need to complete. No story to followĪside from its stunning views, Steep is also blessed by a lack of linear progression. Even choosing the sport-either snowboarding, skiing, wingsuit flying, or paragliding-is as simple as bringing up the menu and choosing one on the fly. With this, you can immerse yourself deeply into the game. Moreover, it can be applied for the screening of FEN1 inhibitors and the monitoring of FEN1 activity in human cells, holding great potential in drug discovery and clinical diagnosis.The technology in the game is so impressive that it allows you to hop around its colossal open world without any loading breaks. This method exhibits good specificity and high sensitivity with a limit of detection (LOD) of 1.75 × 10 −6 U μL −1. The ssRNA can hybridize with a molecular beacon to form an RNA/DNA heteroduplex that can be selectively digested by DSN to generate an enhanced fluorescence signal. Upon the addition of T7 RNA polymerase, an efficient T7 transcription amplification reaction is initiated to produce abundant single-stranded RNAs (ssRNAs). The ssDNA can hybridize with the T7 promoter-bearing template probe to trigger the extension with the aid of Klenow fragment (KF) DNA polymerase. In the presence of FEN1, the flapped dumbbell probe is cleaved to generate a free 5′ flap single-stranded DNA (ssDNA) with the 3′-OH terminus. Herein, we develop a target-activated T7 transcription circuit-mediated multiple cycling signal amplification platform for monitoring FEN1 activity in cancer cells. The structure-specific endonuclease flap endonuclease 1 (FEN1) is an essential functional protein in DNA replication and genome stability, and it has been identified as a promising biomarker and drug target for multiple cancers.
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